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human normal liver cell line lo2  (ATCC)


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    ATCC human normal liver cell line lo2
    Human Normal Liver Cell Line Lo2, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+liver+lo2+cells/Streptomyces+alboniger+Porter+et+al/pmc11239823__41467_2024_50202_MOESM1_ESM-44-0-6
    Average 94 stars, based on 50 article reviews
    human normal liver cell line lo2 - by Bioz Stars, 2026-09
    94/100 stars

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    Activity Assay:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    Colorimetric Assay:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    Fractionation:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    Mutagenesis:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    Planar Chromatography:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    shRNA:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    Quantitative RT-PCR:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    Recombinant:

    Article Title: Mitochondrial SENP2 regulates the assembly of SDH complex under metabolic stress.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER TRIzol Invitrogen AM9738 LipofectamineTM 2000 Invitrogen 11668019 PrimeSTAR Max DNA Polymerase Takara R045A DMEM (No glucose) Gibco 11966025 DMEM (No glutamine) Gibco 11960044 N-Ethylmaleimide (NEM) Sigma-Aldrich E3876; CAS:128-53-0 Acetylcysteine (NAC) Sigma-Aldrich 1009005; CAS:616-91-1 NativeMarkTM Unstained Protein Standard Invitrogen LC0725 MitoSOXTM Red mitochondrial superoxide indicator Invitrogen M36008 5(6)-carboxy-2’,7’-dichlorofluorescein(CDCF) AAT Bioquest AAT-235;CAS: 111843-78-8 Critical commercial assays Succinate Dehydrogenase Activity Colorimetric Assay Kit BioVision Cat#K660-100 Fumarate Colorimetric Assay Kit BioVision Cat#K633-100 Succinate Colorimetric Assay Kit BioVision Cat#K649-100 Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S Mitochondria/Cytosol Fractionation Kit BioVision Cat#K256-100 QuikChange site-directed mutagenesis kit Agilent Cat#200519 Experimental models: Cell lines Human embryonic kidney cell line HEK293T ATCC CRL-3216 Human liver cancer cell HepG2 ATCC HB-8065 Human liver cancer cell Huh7 Shanghai Cell Bank (CAS, Shanghai, China) TCHu182 Human hepatocyte cell LO2 Shanghai Cell Bank (CAS, Shanghai, China) GNHu 6 Human liver cancer cell PLC/PRF/5 ATCC CRL-8024 Experimental models: Organisms/strains BALB/c Nude Mouse Shanghai SLAC Laboratory Animal Co.,Ltd N/A C57BL/6 Shanghai SLAC Laboratory Animal Co.,Ltd N/A Oligonucleotides shRNA sequences, see Table S2 This paper N/A Primers for sgRNA-resistant pCDH-SDHA, see Table S2 This paper N/A Primers used in qRT-PCR analysis, see Table S2 This paper N/A Primers for site-directed mutagenesis, see Table S2 This paper N/A Recombinant DNA p3xFLAG-SDHA WT This paper N/A p3xFLAG-SDHA K20R This paper N/A p3xFLAG-SDHA K41/K46R This paper N/A p3xFLAG-SDHA K92R This paper N/A p3xFLAG-SDHA K128R This paper N/A p3xFLAG-SDHA K167R This paper N/A p3xFLAG-SDHA K179/K182R This paper N/A p3xFLAG-SDHA K250R This paper N/A p3xFLAG-SDHA K335R This paper N/A p3xFLAG-SDHA K361R This paper N/A (Continued on next page) 20 Cell Reports 42, 112041, February 28, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER HA-CBP Gift from Dr. Yichuan Xiao (Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences) N/A pCMV-HA-GCN5 This paper N/A pCMV-HA-PCAF This paper N/A pCMV-HA-TIP60 This paper N/A sh-CBP-1 This paper N/A sh-CBP-2 This paper N/A Software and algorithms GraphPad Prism 5 GraphPad Software https://www.graphpad.com

    other:

    Article Title: VB12-Sericin-PBLG-IR780 Nanomicelles for Programming Cell Pyroptosis via Photothermal (PTT)/Photodynamic (PDT) Effect-Induced Mitochondrial DNA (mitoDNA) Oxidative Damage.
    Article Snippet: Pyroptosis, a kind of programmed cell death involving inflammation, might be a powerful way to fight against tumors, for example, using immunotherapy.. However, how to trigger pyroptosis in cancer cells is an important issue.. Photothermal (PTT)/photodynamic (PDT) therapy is a crucial strategy for inducing cancer cell pyroptosis with noninvasiveness.



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    ScienCell human normal liver cells (lo2)
    Impact of siNCOA4 on cellular responses in <t>LO2</t> cells. A: NCOA4 mRNA expression in LO2 cells after siNCOA4 transfection (detected by RT-PCR); silencing efficiency was verified ( P < 0.01). B: NCOA4 protein expression in LO2 cells after siNCOA4 transfection (detected by Western Blot); silencing efficiency was confirmed ( P = 0.03). C: Cell viability in control, siNCOA4, LPS, and LPS + siNCOA4 groups (assessed by CCK-8 assay); siNCOA4 reversed LPS-induced viability reduction ( P = 0.020, one-way ANOVA). D: Mitochondrial morphology in each group (observed by transmission electron microscopy, scale bar: 10 μm); siNCOA4 alleviated LPS-induced mitochondrial deformation. E: Intracellular Fe²⁺ levels (detected by FerroOrange staining, scale bar: 100 μm); siNCOA4 reduced LPS-induced Fe²⁺ accumulation ( P = 0.015, one-way ANOVA). F: Mitochondrial membrane potential (assessed by JC-1 fluorescence, scale bar: 100 μm); siNCOA4 restored LPS-reduced membrane potential ( P = 0.018, one-way ANOVA). n = 5.
    Human Normal Liver Cells (Lo2), supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+liver+lo2+cells/human+normal+liver+cell+line+lo2/pm38668526-102-9-17
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    human normal liver cells (lo2) - by Bioz Stars, 2026-09
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    lo2 human normal liver cells  (iCell Bioscience Inc)
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    iCell Bioscience Inc lo2 human normal liver cells
    Impact of siNCOA4 on cellular responses in <t>LO2</t> cells. A: NCOA4 mRNA expression in LO2 cells after siNCOA4 transfection (detected by RT-PCR); silencing efficiency was verified ( P < 0.01). B: NCOA4 protein expression in LO2 cells after siNCOA4 transfection (detected by Western Blot); silencing efficiency was confirmed ( P = 0.03). C: Cell viability in control, siNCOA4, LPS, and LPS + siNCOA4 groups (assessed by CCK-8 assay); siNCOA4 reversed LPS-induced viability reduction ( P = 0.020, one-way ANOVA). D: Mitochondrial morphology in each group (observed by transmission electron microscopy, scale bar: 10 μm); siNCOA4 alleviated LPS-induced mitochondrial deformation. E: Intracellular Fe²⁺ levels (detected by FerroOrange staining, scale bar: 100 μm); siNCOA4 reduced LPS-induced Fe²⁺ accumulation ( P = 0.015, one-way ANOVA). F: Mitochondrial membrane potential (assessed by JC-1 fluorescence, scale bar: 100 μm); siNCOA4 restored LPS-reduced membrane potential ( P = 0.018, one-way ANOVA). n = 5.
    Lo2 Human Normal Liver Cells, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+liver+lo2+cells/l02+cells/pm38619892__ic4c00215_si_001-104-16-25
    Average 90 stars, based on 1 article reviews
    lo2 human normal liver cells - by Bioz Stars, 2026-09
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    Impact of siNCOA4 on cellular responses in LO2 cells. A: NCOA4 mRNA expression in LO2 cells after siNCOA4 transfection (detected by RT-PCR); silencing efficiency was verified ( P < 0.01). B: NCOA4 protein expression in LO2 cells after siNCOA4 transfection (detected by Western Blot); silencing efficiency was confirmed ( P = 0.03). C: Cell viability in control, siNCOA4, LPS, and LPS + siNCOA4 groups (assessed by CCK-8 assay); siNCOA4 reversed LPS-induced viability reduction ( P = 0.020, one-way ANOVA). D: Mitochondrial morphology in each group (observed by transmission electron microscopy, scale bar: 10 μm); siNCOA4 alleviated LPS-induced mitochondrial deformation. E: Intracellular Fe²⁺ levels (detected by FerroOrange staining, scale bar: 100 μm); siNCOA4 reduced LPS-induced Fe²⁺ accumulation ( P = 0.015, one-way ANOVA). F: Mitochondrial membrane potential (assessed by JC-1 fluorescence, scale bar: 100 μm); siNCOA4 restored LPS-reduced membrane potential ( P = 0.018, one-way ANOVA). n = 5.

    Journal: Scientific Reports

    Article Title: HNF4A ameliorates acute liver failure by inhibiting NCOA4-mediated ferritinophagy

    doi: 10.1038/s41598-025-13798-3

    Figure Lengend Snippet: Impact of siNCOA4 on cellular responses in LO2 cells. A: NCOA4 mRNA expression in LO2 cells after siNCOA4 transfection (detected by RT-PCR); silencing efficiency was verified ( P < 0.01). B: NCOA4 protein expression in LO2 cells after siNCOA4 transfection (detected by Western Blot); silencing efficiency was confirmed ( P = 0.03). C: Cell viability in control, siNCOA4, LPS, and LPS + siNCOA4 groups (assessed by CCK-8 assay); siNCOA4 reversed LPS-induced viability reduction ( P = 0.020, one-way ANOVA). D: Mitochondrial morphology in each group (observed by transmission electron microscopy, scale bar: 10 μm); siNCOA4 alleviated LPS-induced mitochondrial deformation. E: Intracellular Fe²⁺ levels (detected by FerroOrange staining, scale bar: 100 μm); siNCOA4 reduced LPS-induced Fe²⁺ accumulation ( P = 0.015, one-way ANOVA). F: Mitochondrial membrane potential (assessed by JC-1 fluorescence, scale bar: 100 μm); siNCOA4 restored LPS-reduced membrane potential ( P = 0.018, one-way ANOVA). n = 5.

    Article Snippet: The Human LO2 liver cell line (CL-0111, Procell Life Science & Technology Co., Ltd.) was maintained in RPMI-1640 medium (PM150110, Procell) supplemented with 10% fetal bovine serum (FBS, 164210-500, Gibco) and 1% penicillin/streptomycin (P/S, PB180120 , Procell) at 37 °C in a humidified atmosphere containing 5% CO2.

    Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, CCK-8 Assay, Transmission Assay, Electron Microscopy, Staining, Membrane, Fluorescence

    Cellular Impact of HNF4A Overexpression (oeHNF4A) in LO2 Cells. A: Cell viability in control, oeHNF4A, LPS, and LPS + oeHNF4A groups (assessed by CCK-8 assay); oeHNF4A reversed LPS-induced viability reduction ( P = 0.025, one-way ANOVA). B: Mitochondrial morphology in each group (observed by transmission electron microscopy, scale bar: 10 μm); oeHNF4A alleviated LPS-induced mitochondrial deformation. C: Intracellular Fe²⁺ levels (detected by FerroOrange staining, scale bar: 100 μm); oeHNF4A reduced LPS-induced Fe²⁺ accumulation ( P = 0.017, one-way ANOVA). D: Mitochondrial membrane potential (assessed by JC-1 fluorescence, scale bar: 100 μm); oeHNF4A restored LPS-reduced membrane potential ( P = 0.019, one-way ANOVA). n = 5.

    Journal: Scientific Reports

    Article Title: HNF4A ameliorates acute liver failure by inhibiting NCOA4-mediated ferritinophagy

    doi: 10.1038/s41598-025-13798-3

    Figure Lengend Snippet: Cellular Impact of HNF4A Overexpression (oeHNF4A) in LO2 Cells. A: Cell viability in control, oeHNF4A, LPS, and LPS + oeHNF4A groups (assessed by CCK-8 assay); oeHNF4A reversed LPS-induced viability reduction ( P = 0.025, one-way ANOVA). B: Mitochondrial morphology in each group (observed by transmission electron microscopy, scale bar: 10 μm); oeHNF4A alleviated LPS-induced mitochondrial deformation. C: Intracellular Fe²⁺ levels (detected by FerroOrange staining, scale bar: 100 μm); oeHNF4A reduced LPS-induced Fe²⁺ accumulation ( P = 0.017, one-way ANOVA). D: Mitochondrial membrane potential (assessed by JC-1 fluorescence, scale bar: 100 μm); oeHNF4A restored LPS-reduced membrane potential ( P = 0.019, one-way ANOVA). n = 5.

    Article Snippet: The Human LO2 liver cell line (CL-0111, Procell Life Science & Technology Co., Ltd.) was maintained in RPMI-1640 medium (PM150110, Procell) supplemented with 10% fetal bovine serum (FBS, 164210-500, Gibco) and 1% penicillin/streptomycin (P/S, PB180120 , Procell) at 37 °C in a humidified atmosphere containing 5% CO2.

    Techniques: Over Expression, Control, CCK-8 Assay, Transmission Assay, Electron Microscopy, Staining, Membrane, Fluorescence